16S E. coli rRNA expression construct?

Robert H. Gross Robert.H.Gross at dartmouth.edu
Tue Dec 22 12:58:17 EST 1992


Hi folks,

I am interested in obtaining a construct that has an in vitro
utilizable promoter (e.g. T7 or SP6) upstream of an E. coli 16S rRNA
"gene". The idea is to transcribe copies of the RNA in vitro. Does
anyone have such a beast they would be willing to share or know of a
place I might obtain it? If not in an expression vector, how about a
construct with the 16S region that I could transfer into an expressin
vector?

Thanks in advance and have a happy holiday season.

Bob Gross
Dept. of Biology, Dartmouth College
bob.gross at dartmouth.edu



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