? How to destroy DNA chemically (to reuse DNA columns)
Dennis J. Templeton
djt2 at po.CWRU.Edu
Wed Dec 23 14:34:52 EST 1992
In a previous article, bl275 at cleveland.Freenet.Edu (Dan Diaz) says:
>In a previous article, djt2 at po.CWRU.Edu (Dennis J. Templeton) says:
>>Well, the old cheapskate (me) has broken down and bought a bunch of
>>silica-based columns for DNA column purification (plasmid). We went with
>>those from Machery Nagel (Nucleobond) bought in the US from The Nest Group.
>>These are similar to the Qiagen columns, but the Nest Technical support was
>>more informative and they were a little cheaper.
>I am confused. Qiagen columns are DEAE anion exchangers and yet you say
>they are similar to a silica column?
Both columns are anion exchangers (subtly different) on a silica matrix.
>Second point: sounds like you are wasting time and effort and taking a
>potentially hazardous (in time and money) risk in trying to re-use columns.
>Qiagen columns are not *that* expensive. Spend the extra money and sleep
>well at night. Sure as hell beats troubleshooting.
You have a point, but our use here will consume ca $6000 per year. Here's
a comparison: would you throw away 10 FPLC columns a year at $600 each to
sleep better at night? maybe you or some would. This is a variation of
the "kit mentality" theme discussed here several times. I think there are
valid arguments on both sides of the discussion.
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