labelling dog muscle cells (primary culture)
BIOSEE at UKCC.UKY.EDU
Wed Dec 23 11:06:17 EST 1992
As an infrequent reader of this group, I don't know if this question is
appropriate in this context. If not, I'm sorry and where should I post
Here's my problem. I am supposed to label a primary dog muscle cell
culture so that the cells can be recognized when they are reinjected
in the same dog.
1. Is it feasable (sp?) to use a marker-plasmid to do that or
are the cells going to be senescent by the time I have transfected
and selected them? (I have never worked with primary cell culture)
2. Should I go to a retroviral vector? They are supposed to be pretty
selective in their host. What vectors could be used in a dog?
3. Where could I find information of this kind? Not only the above
questions, but things like "general" transfection procedures etc.
I realize that these questions are very general and there's probably
some easy place to find all this. So,please excuse my ignorance. I am
new to this.
Please e-mail replies since I don't have access to the net very often.
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