supercoiled DNA migration in agrose ?

chai_z at wehi.edu.au chai_z at wehi.edu.au
Tue Dec 22 20:11:36 EST 1992


Hi,

	Several batches of M13 RF DNA of mine have been cut by EcoRI or SmaI,
etc. and then electrophoresized in agrose gel. Uncut DNA (supercoiled) of some
batches migrated faster than the cut one (linear), while the others slower.
Most likely, some batches of my DNA were nicked (open circular). Could any one
tell me the correct agrose pattern of supercoiled, open circular and linear DNA?
or tell me where to refer to?

Thanks in advance.


Zhonglin Chai 



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