Plant RNA Isolation Methods

BOB AT COMB belas at comb1.comb.umd.edu
Mon Dec 28 09:42:00 EST 1992


	I have need of a method of isolating and purified total RNA from cells
with lots of polysaccharides which co-purify with RNA (when using routine
protocols.)  Although the cells I am working with are bacterial, I would like
to try one of the plant RNA protocols on these cells.  My reasoning is that
since many of the protocols for plant cells are especially designed to deal
with such polysaccharides, I might stand a better chance at isolating RNA
from my bacteria.  I would appreciate any suggestions and references to
such protocols that deal with extraction of RNA from high polysaccharide 
backgrounds.  Thanks.

	Bob


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~~    "By tomorrow, a pot smoker will forget he ever read this message."     ~~
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~~                     Bob (Belas at mbimail.umd.edu)                           ~~
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