Methods December 1992 Archives by date

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Starting: Tue Dec 1 02:27:27 EST 1992
Ending: Wed Dec 30 14:40:03 EST 1992
Messages: 280

Random priming advice needed David J. Meyer
Random priming advice skspoidn at reading.ac.uk
Random priming advice needed wetsel_r at wums.wustl.edu
Random priming advice skspoidn at reading.ac.uk
lacZ protein fusion vectors BOB AT COMB
M13tv phage problem (In n) Norio Suzuki
Random priming advice needed afc at gnv.ifas.ufl.edu
vertebrate rRNA clone afc at gnv.ifas.ufl.edu
Phosphoimager any experiences? Darren Natale
Apology Darren Natale
Vector databases MURPHY at JII.AFRC.AC.UK
Sequencing Services? maize at bragg.bio.purdue.edu
growth of yeast in EM field Paul Paukstelis
PCR rxns give smears. Any advice? Brain Foley
Vector databases Brain Foley
PEG and blunt end ligations guo at mcclb0.med.nyu.edu
guanidine life span Loryn Sellner
Random priming advice needed Andrew Hobbs
guanidine life span Andrew Hobbs
Extraction of wheat mitochondrial cytochrome c - help. mybdav02 at uctvax.uct.ac.za
Anchor PCR Dr. S. Bhattacharya
vertebrate rRNA clone Dan Jacobson
Vector databases rlippens at ULB.AC.BE
PCR-vector-sequencing DMEIER at mis.mcw.edu
Protein Kinase C Zhiwei Wang
PCR-vector-sequencing wetsel_r at wums.wustl.edu
Kits again!! was Re: Random Priming, etc. John Alsobrook
guanidine life span Pierre Jelenc
Kits again!! was Re: Random Priming, etc. George W Chacko
in vivo crosslinking erman at binah.cc.brandeis.edu
Kits again!! was Re: Random Priming, etc. the End
<None> mic296e at vaxc.cc.monash.edu.au
magic mp & sequenase michael coyne
Kits, right of reply skspoidn at reading.ac.uk
PCR sequencing skspoidn at reading.ac.uk
PCR-vector-sequencing Bruce Roe
Vector databases Bruce Roe
kit flames Rick Wilson
magic mp & sequenase Ashok Aiyar
magic mp & sequenase Bruce Roe
Kits again!! was Re: Random Priming, etc. Ernest Retzel 1535 49118
magic mp & sequenase wetsel_r at wums.wustl.edu
magic mp & sequenase wmelchior at ntet.nctr.fda.gov
guanidine life span Pierre Jelenc
magic mp & sequenaseDIR wmelchior at ntet.nctr.fda.gov
Kits again!! was Re: Random Priming, etc. Jarmo Niemi TUY
TB1 (E.Coli) Any Info Helpful dnicker at vax.oxford.ac.uk
northern blot Ed Rybicki
DNA polymerase Ting H. Hsiao
magic mp & sequenase wmelchior at ntet.nctr.fda.gov
Hygromycin toxicity BMA14432 at VAX1.UTULSA.EDU
Vent of Pfu - which is more HiFi ? kucera at neb.com
magic mp & sequenase Paul N Hengen
Anchor PCR Martin Kennedy
Vector databases cherry at OPAL.MGH.HARVARD.EDU
Hygromycin toxicity Brain Foley
reply Jaishree Muppala Chittoor
reply to guanidinium thiocyanate Jaishree Muppala Chittoor
re random priming J Preiss--Seq Anal
NATO ADVANCED STUDY INSTITUTE TAYLAN
TB1 (E.Coli) Any Info Helpful Michael Benedik
northern blot Ed Rybicki
Replacement of Urea in Sequencing Gels sensen at embl-heidelberg.de
Rep of U. Mark II sensen at embl-heidelberg.de
magic mp & sequenase gagnon at vax.ox.ac.uk
cotransfection with oct1 help or suggestions needed IBELGAUFTS at vms.biochem.mpg.de
methylomonas clara contacts wanted STIEBLER at vms.biochem.mpg.de
magic mp & sequenaseDIR Bruce Roe
magic mp & sequenase Hugo Eric
probes for LDH ? LERCH
TB1 (E.Coli) Any Info Helpful Earl Edwin Rutenber
magic mp & sequenase Cheung C. Yue
supplier of spin-"filters" Basavaraju Shankarappa
oligodT column matrices DMEIER at mis.mcw.edu
Beta-gal cotransfection - nonsense results? Jorge L. Sepulveda
Betagen sequencers twomey at helix.nih.gov
Immunopreciptation Wun Chey Sin
TB1 (E.Coli) Any Info Helpful GIETZ at BLDGHSC.LAN1.umanitoba.ca
Vent of Pfu - which is more HiFi ? Ashok Aiyar
HighFive cells- Are they worth it? Clifford J Beall
Vector databases David Kristofferson
getting rid of dye terminatorsABI Beth Stewart
In Situ PCR David A. Adler
magic mp & sequenase gagnon at vax.ox.ac.uk
Thanks for TB1 Info!!! ==|:) dnicker at vax.oxford.ac.uk
kit flames jasper at vax.path.ox.ac.uk
PEG ligations summary... George W Chacko
Methods for anchored PCR/RACE Martin Kennedy
kit flames George W Chacko
HighFive cells- Are they worth it? Clifford J Beall
Advice on video of Ethidium Bromide gels needed. Gus Trautweiler
FM3A cells supplier wanted HELP IBELGAUFTS at vms.biochem.mpg.de
Lowry Protein Measurement Jen
Lowry Protein Measurement BOB AT COMB
LANL/Genbank Vector database available for searching via Gopher Dan Jacobson
Non-radioactive detection systems wetsel_r at wums.wustl.edu
MC1061/P3 minipreps... wetsel_r at wums.wustl.edu
Immunopreciptation vwalther at ulys.unil.ch
FM3A cells supplier wanted HELP Alan Gerstein
Non-radioactive detection systems David Nunn
Immunopreciptation DMEIER at mis.mcw.edu
Re-immunoprecipitation NI,WEITING
MC1061/P3 minipreps... Basavaraju Shankarappa
Subcloning Poly-Linkers J Preiss--Seq Anal
DNA-binding protein cloning Wei Shao", ADDRESS: IN%"w_shao at uvmvax.uvm.edu
LARGE PLASMIDS lyons at wehi.edu.au
DNA from Polyacrylamide & Protein Fusion and Purification System Michael CHENG
non-radioactive DNA detection Ed Rybicki
Protein Fusion and Purification System NANTEL at UNCVX1.BITNET
Advice on video of Ethidium Bromide gels needed. Ross Whetten
Duodenal promoter wanted bullock at lincoln.ac.nz
Rabbit genomic library bullock at lincoln.ac.nz
Nonradioactive In situs? U22379 at UICVM.UIC.EDU
site-directed mutagenesis problems Fiberman
Restriction near DNA ends DMEIER at mis.mcw.edu
Peak Wavelength of EtBr stained DNA under UV? Bradley K. Sherman
Any info about the new peptide synthesizer Synergy from ABI??? Bo Servenius
DNA from Polyacrylamide & Protein Fusion and Purification System Basavaraju Shankarappa
Agarose gel migration artifacts of PCR products. Basavaraju Shankarappa
Molecular Modelling Peter Ruben
DNA from Polyacrylamide Gels Ashok Aiyar
MC1061/P3 minipreps... Bryan J. Maloney
site-directed mutagenesis problems Russell A. Maurer
genomic library Jaishree Muppala Chittoor
Restriction near DNA ends Martin Kennedy
MJ PCR machines HRMBDKC at HKUMD1.HKU.HK
Expression vectors Andy Phillips
Rabbit genomic library wetsel_r at wums.wustl.edu
(none) Dr Huaizheng Peng
pcr primers suter at VAX.MPIZ-KOELN.MPG.dbp.de
Protein Fusion and Purification System Mark Bradley
LARGE PLASMIDS Bruce Roe
DNA from Polyacrylamide Gels Bruce Roe
5' RACE after RNA ligase - reference needed Cheung C. Yue
Protein Fusion and Purification System NANTEL at UNCVX1.BITNET
re genomic library J Preiss--Seq Anal
[No subject] Bouchier ChristianeBouchier ChristianeBouchier Christiane
Sequencing Problem... wetsel_r at wums.wustl.edu
PCR enzymes... wetsel_r at wums.wustl.edu
northern blot Euan R. Taylor
Cleaning electroporation cuvettes David Nunn
PCR-vector-sequencing Stuart Brown
Vent of Pfu - which is more HiFi ? Stuart Brown
(none) Denis Tang
Peak Wavelength of EtBr stained DNA under UV? Ross Whetten
Advice on video of Ethidium Bromide gels needed. Ross Whetten
Agarose gel migration artifacts of PCR products. Dennis J. Templeton
Expression vectors Dennis J. Templeton
Suggestion: Put your question into the Subj: line please. Dennis J. Templeton
need advice on PC software Bryan Crawford
met7 mutant at ccwf.cc.utexas.edu, Leon F. Garcia
Vent and exonuclease activity (was Re: PCR enzymes...) Ashok Aiyar
Switching over salim at mindi.mibi.su.se
Sequencing Problem... Mart Speek
recovery of proteins from phenol Agustin Vioque
amp gene with internal tga stop mutations wanted STIEBLER at genmic
suppliers of ampicillin antibodies wanted STIEBLER at genmic
recovery of proteins from phenol Bruce Roe
Sequencing Problem... BOB AT COMB
Cleaning electroporation cuvettes Dubear Kroening
Cleaning electroporation cuvettes BOB AT COMB
need advice on PC software CZJ at CU.NIH.GOV
recovery of proteins from phenol roy french
Amp-r plasmids with defective lacZ gene Basavaraju Shankarappa
LARGE PLASMIDS the End
recovery of proteins from phenol Basavaraju Shankarappa
Proteins from phenol Ed Rybicki
IgM for affinity chromatography NGUYENHD
Immuno-affinity Chromatography Basavaraju Shankarappa
m rna extraction from tissue DMEIER at mis.mcw.edu
How to empower RNases?! DMEIER at mis.mcw.edu
5' RACE after RNA ligase - reference needed Cheung C. Yue
(none) pakapke at IASTATE.EDU
Help: Panning JnP
Agarose gel migration artifacts of PCR products. Mark Schweder
sequence and/or restriction map of EMBL4 Donald Chen - Microbiology
(none) C. S. Enderlin
Linker scanning summary Denis Tang
Cleaning electroporation cuvettes Anthony Davies
Help: Panning GARBRECHT
electroporation pakapke at IASTATE.EDU
Summary: Non-radioactive detection of DNA David Nunn
low Tm primer PCR Zhengyu Sha
amp gene with internal tga stop mutations wanted Keith Robison
ENZO rpgrant at vax.path.ox.ac.uk
Taq - not so promiscuous??? rpgrant at vax.path.ox.ac.uk
restriction map of EMBL4 afc at gnv.ifas.ufl.edu
Dig Southern Blocking Dave KNecht
electroporation (to make cDNA libraries) Dennis J. Templeton
3' overlaps in PCR DMEIER at mis.mcw.edu
site directed mutagenesis problems GIETZ at bldghsc.lan1.umanitoba.ca
MC1061/P3 minipreps GIETZ at bldghsc.lan1.umanitoba.ca
Subcloning Poly-Linkers GIETZ at bldghsc.lan1.umanitoba.ca
Cleaning electroporation cuvettes Martin Kennedy
Cleaning electroporation cuvettes forsburg at vax.path.ox.ac.uk
PIPES Nir Navot
electroporation (to make cDNA libraries) Rex L. Chisholm
Mobility Shift Assay Nir Navot
ENZO Paul Walden
Need source for polyclonal anti-human collagen IV Vivek Kapur
Expression vectors Andy Phillips
Taq - not so promiscuous??? Robert Horton
low Tm primer PCR steven heckt
colony screening with Amersham ECL (RPN 3000 and 3004) Christiane Bouchier
3' overlaps in PCR Brain Foley
Synthetic oligo with "blocked" 3'-OH group Cheung C. Yue
Taq - not so promiscuous??? bhjelle at carina.unm.edu
signoff MNAVARRE at ANDESCOL.bitnet
NON_RADIOACTIVE NORTHERNS- personal summary Euan R. Taylor
Summary: Phosphoimager experiences Joerg Sprengel
Thanks for random priming advice tronni at nphi.fi
Cleaning electroporation cuvettes Klaus.Matthaei at anu.edu.au
Mobility Shift Assay Jorge L. Sepulveda
Q: fusion protein expressin Zhao Zhuoshen Mr
DNA from Polyacrylamide & Protein Fusion and Purification System Victor Jongeneel
MHC T-cell epitopes vladimir at wehi.edu.au
colony screening with Amersham ECL (RPN 3000 and 3004) afc at gnv.ifas.ufl.edu
Removing RNA from a mixture of protein and RNA Basavaraju Shankarappa
Plant protein isolation BOB MCNULTY
Acid washed sand LAKSHMAN at MAINE.MAINE.EDU
Live Bgal Detection: SUMMARY Richard S Sucgang
Q: fusion protein expressin GIETZ at bldghsc.lan1.umanitoba.ca
automatic DNA sequencing systems HRMBDKC at HKUMD1.HKU.HK
Acid washed sand Bruce Roe
colony screening with Amersham ECL (RPN 3000 and 3004) bhjelle at carina.unm.edu
DNA from Polyacrylamide Gels Paul N Hengen
Mobility Shift Assay Harry Mangalam
protein seq. alignment programe Sudhanshu Vrati
Mobility Shift Assay DMEIER at mis.mcw.edu
Q: fusion protein expressin greenleaf at bchm.biochem.duke.edu
Mobility Shift Assay Harry Mangalam
protein seq. alignment programe Dan Jacobson
IgM for affinity chromatography GARBRECHT
Removing RNA from a mixture of protein and RNA Christo Houbaviy
colony screening with Amersham ECL (RPN 3000 and 3004) USENET News System
Acid washed sand Andrew Hobbs
(none) URA1335 at FRCITI51.BITNET
T-tracking program ? Jarmo Niemi TUY
Horseradish Peroxidase Blots Heidi Lanora Hinkel
Mobility Shift Nir Navot
Acid washed sand Paulo Magalhaes
Fast DNA from muscle biopsies Paulo Magalhaes
gene synthesis Ding Ming
Commercial peptide sources DMEIER at mis.mcw.edu
(none) pakapke at IASTATE.EDU
None (HPRT Northerns) Martin Kennedy
Request for tobacco seeds information u6069416 at ucsvc.ucs.unimelb.edu.au
T-tracking program ? parkhillj at vax1.bham.ac.uk
T-tracking program ? Victor Jongeneel
Pulsed Field Electrophoresis bishop at MAP.MARC.USDA.GOV
? How to destroy DNA chemically (to reuse DNA columns) Dennis J. Templeton
16S E. coli rRNA expression construct? Robert H. Gross
Sequencing Primers for Automated Sequencing Philip L. Carl
supercoiled DNA migration in agrose ? chai_z at wehi.edu.au
? How to destroy DNA chemically (to reuse DNA columns) Dan Diaz
destroying dna with DEPC suter at VAX.MPIZ-KOELN.MPG.dbp.de
labelling dog muscle cells (primary culture) Stephanie Edelmann
labelling dog muscle cells (primary culture) David Steffen
T-Tracking etc. Dr. S. Bhattacharya
? How to destroy DNA chemically (to reuse DNA columns) Dennis J. Templeton
? How to destroy DNA chemically (to reuse DNA columns) Dan Diaz
Sequencing Primers for Automated Sequencing Bruce Roe
Seeking psoralen tethered oligos SEGAL, DAVID JAY
MEDLINE Gabriel Trueba
MEDLINE Ashok Aiyar
MEDLINE Tao Tao
supercoiled DNA migration in agrose ? Martin Kennedy
MEDLINE Don Gilbert
MEDLINE David Kristofferson
MEDLINE David Kristofferson
supercoiled DNA migration in agrose ? Victor Jongeneel
Plant RNA Isolation Methods BOB AT COMB
supercoiled DNA migration in agrose [TBE vs. TAE or TPE gels] Dennis J. Templeton
? How to destroy DNA chemically (to reuse DNA columns) Michael Benedik
supercoiled DNA migration in agrose [TBE vs. TAE or TPE gels] Dennis J. Templeton
Plasmid DNA Extraction Lixin Zhou
Lorist 6 vector information William J. Buikema
supercoiled DNA migration in agrose [TBE vs. TAE or TPE gels] afc at gnv.ifas.ufl.edu
Plasmid DNA Extraction Peter M. Muriana
Plasmid DNA Extraction Lixin Zhou

Last message date: Wed Dec 30 14:40:03 EST 1992
Archived on: Sun Mar 6 19:46:00 EST 2005

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