RT-PCR

Dr. Art Buchberg buchberg at CALVIN.JCI.TJU.EDU
Tue Feb 25 19:13:54 EST 1992


Hi netters
I would like to poll the community on their favorite protocol for RT-PCr.
IS it better to do the RT with specific oligos or oligo-dT?
How to adjust conditions to get a quantitative system for determining
levels of transcripts for a variety of mRNAs that have very different levels
of expression?
And any other general (or specific advice) that people would like to contribute, or pitfalls that others have experienced.
ANy and all advice is welcome
	and a compendium of all advice will be submitted to the NET
THANKs in advance
Art




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