Bind silane protocols for seq gels

B.J. camlau at ubvmsb.cc.buffalo.edu
Tue Feb 25 16:47:00 EST 1992


In article <1992Feb25.141537.935 at uwovax.uwo.ca>, 37_527 at uwovax.uwo.ca (Gregory A. Denomme) writes...
>Hello Netters:
>  I would like to obtain receipes for coating the outer plates for sequencing
>gels using BDH's Silane A-174 as the 'bind Silane'.  I am having some trouble
>at the point of separating the plates and maybe a new protocol would help.
> 
>  Thanks in advance
>G
>-- 
>------------------------------------------------------------------
>Gregory Denomme     email: gdenomme at uwovax.uwo.ca
>University Hospital - BRE12
>PO Box 5339
>London, Ontario, CANADA N6A 5A5
>Tel: (519)663-3551 FAX:(519)663-3743

Our lab uses PAM to help gels unstick.  That's right, PAM, the vegetable
spray shortening.  Seems to work wonders.  Just spray on, wipe down excess.

Joseph Lau
camlau at ubvms.cc.buffalo.edu



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