Bind silane protocols for seq gels

B.J. camlau at
Tue Feb 25 16:47:00 EST 1992

In article <1992Feb25.141537.935 at>, 37_527 at (Gregory A. Denomme) writes...
>Hello Netters:
>  I would like to obtain receipes for coating the outer plates for sequencing
>gels using BDH's Silane A-174 as the 'bind Silane'.  I am having some trouble
>at the point of separating the plates and maybe a new protocol would help.
>  Thanks in advance
>Gregory Denomme     email: gdenomme at
>University Hospital - BRE12
>PO Box 5339
>London, Ontario, CANADA N6A 5A5
>Tel: (519)663-3551 FAX:(519)663-3743

Our lab uses PAM to help gels unstick.  That's right, PAM, the vegetable
spray shortening.  Seems to work wonders.  Just spray on, wipe down excess.

Joseph Lau
camlau at

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