RNase treatment of DNA

IYAP at IRRI.CGNET.COM IYAP at IRRI.CGNET.COM
Thu Feb 20 00:57:45 EST 1992


Is it necessary to treat DNA with RNase if it's going to
be used for Southern blotting?  Our standard protocol calls
for RNase treatment during extraction.  We've had some
reports that RNase treatment is NOT needed.  What sort of
interference (if any) would RNA produce in the filter and/or
during hybridization?
Thanks in advance.



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