hybridization incubators

Fri Feb 14 21:17:00 EST 1992

We've been attempting to use a Robbins hybridization incubator for
some time now (glass bottles that rotate).  There seems to an
enormous amount of variability in the quality of the blots we
obtain and it seems to be probe and hybridiation fluid dependent.
Some of the blots have so much background that they cannot be used,
however, the same probe and fluid hybridized in a "seal-a-meal"
bag would look great.  We'ev tried different blotting media as
well with not much success.  Does anyone have a universally
good protocol for this technique that works with all probes?

Marc J. Mass, Ph.D.
MRM at epavax.rtpnc.epa.gov

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