DMSO works!!!

Richard Sucgang rs54 at cunixf.cc.columbia.edu
Fri Feb 14 05:03:18 EST 1992


Whoever suggested that DMSo can increase PCR specificity...
Thanks!!!

I compared the rxn with one that is 10% DMSO, and found a
whopping difference. Whereas I need 80 cycles previously to 
get a product, I get them now at 30. Different bands,too.
Anyway, I have sequenced enugh artifacts.

BTW, the PCR gems from Cetus also have a beneficial effect.


-rich
Richard Sucgang : Dept. of Anatomy and Cell Biology
Columbia University (sucgang at cuhhca.hhmi.columbia.edu; 
de slime god         rs54 at cunixf.cc.columbia.edu)



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