rs54 at cunixf.cc.columbia.edu
Fri Feb 14 05:03:18 EST 1992
Whoever suggested that DMSo can increase PCR specificity...
I compared the rxn with one that is 10% DMSO, and found a
whopping difference. Whereas I need 80 cycles previously to
get a product, I get them now at 30. Different bands,too.
Anyway, I have sequenced enugh artifacts.
BTW, the PCR gems from Cetus also have a beneficial effect.
Richard Sucgang : Dept. of Anatomy and Cell Biology
Columbia University (sucgang at cuhhca.hhmi.columbia.edu;
de slime god rs54 at cunixf.cc.columbia.edu)
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