Radio-labelling EthBr stained DNA

kim at m44.unm.edu kim at m44.unm.edu
Mon Feb 10 12:47:29 EST 1992


In article <1992Feb10.002954.11436 at minyos.xx.rmit.oz.au>, s860780 at minyos.xx.rmit.oz.au (Clinton Grant) writes:
>
>	I am posting this on behalf of a freind:
>	Does anybody know whether DNA taken from a stained gel (and GeneCleaned)
>is OK to label up with P32 (using an oligonucleotide labelling kit)? Does the 
>EthBr interfere with the labelling reaction?
>
>			Thanks in advance. 

I would think the ethanol in the NEW wash should elute most or all of the
Ethidium from the DNA.
Daniel Kim



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