Bill Melchior, NCTR/FDA
wmelchior at NTDOC.NCTNET.GOV
Thu Feb 6 16:31:20 EST 1992
>Does anyone know what is the lower limit of size for precipitable ds or ss
>nucleic acids by trichloroacetic acid? I am only familiar with precipitations
>on a glass fiber filter. Is it possible to do precipitations in solution and
>then simply pellet the material by centrifugation?
The latest issue of BioTechniques (v12 #2, Feb '92) has a note on TCA
precipitation, pp226-228, by KC Gupta. He states that the smallest oligo
that can be measured reliably (my interpretation of his statement) by TCA
pptn + filtration is ~50 ntides. He spots the
solution on filter paper and then immerses the paper in TCA, getting
what appears to be pretty good results down to 10mers.
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