protocol for luciferase assay needed

Tao Tao txt15 at po.CWRU.Edu
Thu Feb 6 15:19:28 EST 1992


I am trying to use the promoterless luciferase plasmid to study the regulation
of the promoter of a gene cloned from Salmonella typhimurium.  However, the 
positive control I constructed was very week and varied in light emission.

Could the experts help me out by providing me with the established protocol
for doing the luciferase assays in liquid as well as solid medium?

Thanks in advance for whatever help that's on the way to me.

Tao



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