Peter M. Muriana
muriana at aclcb.purdue.edu
Mon Feb 3 16:55:42 EST 1992
Since there's been a flurry of PCR-related questions & answers lately,
I thought I'd pose a question towards those who may point out the folly
in my thinking.
If I had a <mixed> DNA probe of <unknown> sequence, such as that
which may be obtained by bacterial genomic subtraction, yet the
amount of subtracted/mixed probe obtained was too little to obtain
sufficient detection using chemiluminescence labelling.
Could this mixed probe be amplified using a PCR adaptation similar
to an oligo-labelling, except with Taq polymerase instead of
Klenow and excess random primers? (even though the product may
not identically represent the proportions of probe present in the
original mixed probe).
Can anyone comment on the feasibility of this working or
why it wouldn't work?
Internet: muriana at aclcb.purdue.edu
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