m5C-DNA cleavage

Dr. Richard Roberts at Cold Spring Harbor Laboratory roberts at CSHL.ORG
Fri Jan 31 16:17:33 EST 1992

ApyI is not specific for m5C residues!  The enzyme is very similar to
BstNI.  ie it will cut m5C-containing DNA if the m5C is at the inner cytosine.
This is almost certainly because it`s own modification system uses m4C .

Rich Roberts

More information about the Methods mailing list