5mC insensitive restriction enzyme
RSUNDARP at cipvax.biolan.Uni-Koeln.DE
Fri Jan 31 08:29:11 EST 1992
In <A9DF7F679E9F404927 at twnas886.bitnet> R303 at imb.as.tw writes:
> Hi netters,
> Does anyone out there know any restriction enzymes which only cut
> 5mC-substituted DNA but not normal-based DNA?
> Please sent to: "R303 at imb.as.tw"
> Thanks in advance for any information.
> Institute of Molecular Biology
> Academic Sincia
> Taipei, Taiwan, ROC
there is a vector-system based on restriction activity against 5mC-
containing DNA (Noyer-Weidner and Reiners-Schramm, Gene, 66 (1988) 269-278)
They use rglB-nuclease in combination with cloning sites in a ts-Methylase.
In this paper they also metion rglA nuclease cutting CC*GG, whereas rglB
cuts at GGC*C and C*C*GG.
ButoeBut I have never heard about obtaining commercially purified rglA or rglB.
Hope I could help you
More information about the Methods