Hirt Supernatents for Cloning Viral DNA

Brian Hjelle bhjelle at vaxine.unm.edu
Tue Jan 28 19:10:28 EST 1992


I am planning to try to clone the episomal form of a retrovirus
using lambda Zap II and a site I expect to be present in the
pol gene. My last efforts on this front flopped.

Does anyone have a protocol for Hirt supernatent preps that
they would recommend? The only paper I have that talks about
this in any detail is the one from Hirt himself (think he
was trying to study T. rex viruses :-) ).

Thanks!

Brian



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