Peter M. Muriana
muriana at aclcb.purdue.edu
Fri Jan 24 10:14:27 EST 1992
Do any of you know of transposable constructs for Gram-positive bacteria
capable of examining gene <<expression>>.
I am looking for something similar to the (promoterless) LacZ gene fusions
or TnphoA systems that have been so useful for examining expression of
virulence-related genes in Gram-negative bacteria.
I know of a host of transposons available for Gram-positive organisms (
Tn916, Tn917, Tn1545, etc.), but I'm looking for one which could be used to
assess expression of the gene into which it is inserted.
If anyone could be of assistance I thank-you.
Internet: muriana at aclcb.purdue.edu
More information about the Methods