Gene Fusions

[Peter M. Muriana]

Dear Bionetters,

Do any of you know of transposable constructs for Gram-positive bacteria 
capable of examining gene <<expression>>.  

I am looking for something similar to the (promoterless) LacZ gene fusions 
or TnphoA systems that have been so useful for examining expression of 
virulence-related genes in Gram-negative bacteria.

I know of a host of transposons available for Gram-positive organisms (
Tn916, Tn917, Tn1545, etc.), but I'm looking for one which could be used to
assess expression of the gene into which it is inserted.

If anyone could be of assistance I thank-you.


    Name: murianap
Internet: muriana at aclcb.purdue.edu
   Phone: 317-494-8284