oligo in situ hybridizations

HRMBDKC at HKUMD1.HKU.HK HRMBDKC at HKUMD1.HKU.HK
Thu Jan 16 05:16:00 EST 1992


I am trying to set up doing in situ hybridization on paraformaldehyde
fixed sections from mouse embryos using a 24mer oligonucleotide.
I would appreciate tips on the best way to do this: end labelling
or A-tailing. 3H or 35S as label. Also the best way to work out
hybridization and washing conditions.
All recipes gratefully received.
Kathy Cheah
HRMBDKC at HKUCC.BITNET



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