I am trying to set up doing in situ hybridization on paraformaldehyde
fixed sections from mouse embryos using a 24mer oligonucleotide.
I would appreciate tips on the best way to do this: end labelling
or A-tailing. 3H or 35S as label. Also the best way to work out
hybridization and washing conditions.
All recipes gratefully received.
HRMBDKC at HKUCC.BITNET