Tet selection problems in pBR322

Pierre Charles Jelenc pcj1 at cunixf.cc.columbia.edu
Sat Jan 11 09:36:17 EST 1992


To induce tet resistance, use a broth containing a small amount of oxy-TC that
was added BEFORE autoclaving. Tetracyclines are mostly destroyed by high
temperatures, but the products still induce the resistance. This is the
basis of the fusaric acid method for selection of tet sensitive mutants.

Pierre

Pierre Jelenc                        pcj1 at cunixf.cc.columbia.edu
Columbia University



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