PCR with primers of different lengths

IO00865 at MAINE.MAINE.EDU IO00865 at MAINE.MAINE.EDU
Wed Jan 8 11:54:41 EST 1992


I am trying to add approx. 60 bp to the 5 prime end of a piece of DNA. Is it
possible to do PCR with one primer of 80 bp (20 overlap and 60 new) and
another of 20 bp (all overlapping). I suspect that their may be problems
with melting after the first few rounds of PCR. Does anyone have any experience
with this kind of thing?
Any suggestions would be appreciated.
Ethan Strauss
IO00865 at maine                              RR#1 Box333
Dept Plant biology and Pathology           Old Town Maine, 04468
Univ. Maine Orono 04469                    207-394-2014



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