What happens to 32P-labelled compounds?

Esa-Pekka P{lvim{ki eepee at polaris.utu.fi
Thu Jul 30 03:58:12 EST 1992


In article <1992Jul27.201324.27564 at access.usask.ca> goldie at herald.usask.ca (Hugh Goldie) writes:
>
>What happens to 32P-isotope labelled compounds when the 32P breaks down?
>I am interested in 32P-gamma-adenosine triphosphate, for example, and
>also oligomeric deoxynucleic acids labelled at their 5' termini with
>32P by the enzyme polynucleotide kinase.
>
>My old radioisotope text (Principals of Radioisotope Methodology, 3rd edn
>Chase G.D. and Rabinowitz, J.L., 1967.) indicates on page 151 that 32P decays
>to 32S by negatron emission and the table in the back of the book indicates 
>that 32S is a stable isotope.  Would the 32S atom merely replace the 32P
>atoms in these molecules?  Would the recoil of beta radiation damage
>adenosine triphosphate or oligonucleotides?

I understand that a S atom in ATP is replaced with a 32P atom to produce a
radioactive ATP molecule. When the 32P decays to 32S, the ATP molecule 
continues to exist as a "real" ATP with a 32S where it should be. 
I might be wrong, though.

/ep



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