Smiles on Southerns

kim at m44.unm.edu kim at m44.unm.edu
Wed Jul 29 13:24:12 EST 1992


In article <1992Jul29.034016.22825 at uniwa.uwa.edu.au>, robert at arbo.microbiol.uwa.oz.au (Robert Coelen) writes:
>In article <d3mmcsa at lynx.unm.edu> kim at m44.unm.edu writes:
>>In article <1992Jul28.110853.24275 at gserv1.dl.ac.uk>, MORRIS at ch.unige.cmu ("Mike Morris, Geneva") writes:
                                    
Stuff about salt and temperature and gel migration deleted
 
IBI makes an electroelution device that works by setting up a high salt "trap"
in which the migration of DNA or proteins is so inhibited that they just stay
in the high salt chamber.  This is a V-shaped tunnel in a piece of Plexiglas in
which a high salt buffer is layered at the bottom, and normal electrophoresis
buffer is layered on top.  The gel slice is placed next to one end of the
tunnel and the current is then applied.  DNA migrates out of the gel slice to
be trapped in the high salt layer.

Does this operate on some other principle than high-salt gel smiles?  Has
anyone tried either the real thing from IBI or a home-made copy?

Daniel Kim



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