Tracer Dyes in Agarose
William_D_WARREN at UMAIL.UMD.EDU
Mon Jul 27 16:43:00 EST 1992
>Hello. For some time I've been using a ficoll based loading
>solution for my agarose gels with both xylene cyanol and
>bromphenol blue added as tracer dyes.
[Stuff deleted] ...So, I ask if anyone knows of
>any other dyes that can be used for this purpose? In particular
>I'm wondering if anyone uses a dye that migrates faster than the
>bromphenol blue front?
>Paul N. Hengen
>National Cancer Institute
>Frederick Cancer Research and Development Center
>Laboratory of Mathematical Biology
I saw a recent article in Biotechniques about the use of alternative
dyes - The context of the article was that the BPB and XCYN, if added
to a PCR reaction prior to amplification will inhibit the reaction.
The study looked at an number of alternative dyes and additives to
increase the solution density and found (as I remember) that it is
possible to add sucrose and one or two of a number of dye alternatives
to a PCR reaction prior to thermocycling and then directly load the
samples onto an agarose gel.
Unfortunately I can't find the article -
but maybe there's another Bioboid out there who can supply the full
reference. Perhaps one of those mentioned in the article will have
the desired mobility that you need.
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