Question on Primer design for Cross-over PCR...
txt15 at po.CWRU.Edu
Fri Jul 24 14:35:03 EST 1992
I would like to get fellow netters opinion on the design of primers for
cross-voer PCR. Specifically, what's the minimum length of overlap? Is
15 bp long enough (with Tm ~45 degree)?
Another question related to this is, what is the "ideal" procedures to
generate the hybrid molecule for next step cross-over PCR? Is it required
or prefered that the two product be made using asymmetric PCR?
Thanks in advance for your reply.
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