Question on Primer design for Cross-over PCR...

Tao Tao txt15 at po.CWRU.Edu
Fri Jul 24 14:35:03 EST 1992

I would like to get fellow netters opinion on the design of primers for
cross-voer PCR.  Specifically, what's the minimum length of overlap? Is
15 bp long enough (with Tm ~45 degree)? 

Another question related to this is, what is the "ideal" procedures to
generate the hybrid molecule for next step cross-over PCR?  Is it required
or prefered that the two product be made using asymmetric PCR?

Thanks in advance for your reply.


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