Bill Melchior, NCTR/FDA
WMELCHIOR at NTET.NCTR.FDA.GOV
Thu Jul 23 14:56:45 EST 1992
A good question -- I'd forgotten about the high temps of PCR.
I've never done it, so I can't answer your question. The important thing
is that it usually seems to work! (Maybe buffer problems are involved in
those cases where it DOESN'T work?)
Maybe I'll find out when I try to do cycle-sequencing, hopefully before long.
The opinions stated are mine, not those of NCTR or its sponsoring organizations.
Bill Melchior || "You have lawyers the way
National Center for Toxicological Research || other people have mice."
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(501) 543-7206 || -Kenneth Duncan, English
|| Health & Safety Executive,
WMELCHIOR at NTDOC.NCTR.FDA.GOV || to US regulators
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