Site directed mutagenesis

Johan deBoer jdboer at SOL.UVIC.CA
Thu Jul 23 00:12:33 EST 1992


Hello,
There is a kit on the market called Transformer for the site directed
mutagenesis of any double stranded plasmid. It uses two mutagenic primers
one to introduce the desired mutation and one to destroy a unique 
restriction site. This way all original plasmid can be destroyed by
restriction digestion.
This looks a very nice method. My question is, has anyone used this
method and does it live up to its promise or is it just another method
like so many others?
I would appreciate any comments from people who have used the kit.
Johan de Boer.



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