Na2HPO4 vs NaH2PO4 in SSPE

DMEIER at mis.mcw.edu DMEIER at mis.mcw.edu
Mon Jul 20 18:17:33 EST 1992


This msg is intended for methods-and-reagents:
Netters,
  When making 20X SSPE for RNA-DNA hybridizations, does it matter if you start
with monobasic or dibasic sodium phosphate since the ionic strength of the buffer differs.  It seems like a silly point but I remember when a grad student lost
one month of work trying to ligate in the presence of TBE instead of TAE!

Dan Meier, MCW
DMEIER at MIS.MCW.EDU




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