PCR problems

Mike Poidinger skspoidn at uk.ac.rdg.susssys1
Mon Jul 20 07:23:29 EST 1992


I have a colleague who is trying to PCR single
 copy genes from eukaryotic DNA by both normal
 and inverse PCR procedures. 30 cycles usually
 results in faint or non-existent bands being 
present at the appropriate sizes. However, when
 these bands are excised and re-PCR'd, then 
numerous smaller fragments are generated, half 
the size and smaller than the original band. Does 
anyone know why, and/or what can be done to prevent it.

Thanks in advance,

Mike Poidinger
Dept of Microbiology
University of Reading

"All opinions stated are someone elses.
 I don't have any of my own"



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