skspoidn at uk.ac.rdg.susssys1
Mon Jul 20 07:23:29 EST 1992
I have a colleague who is trying to PCR single
copy genes from eukaryotic DNA by both normal
and inverse PCR procedures. 30 cycles usually
results in faint or non-existent bands being
present at the appropriate sizes. However, when
these bands are excised and re-PCR'd, then
numerous smaller fragments are generated, half
the size and smaller than the original band. Does
anyone know why, and/or what can be done to prevent it.
Thanks in advance,
Dept of Microbiology
University of Reading
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I don't have any of my own"
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