Home-made PCR gems
Richard S Sucgang
rss19 at cunixa.cc.columbia.edu
Sat Jul 18 14:17:55 EST 1992
In article <01GMHDD8X6MS000A02 at irri.cgnet.com> IYAP at IRRI.CGNET.COM writes:
>Our lab happened to have several bags of Paraplast (tissue embedding
>medium) lying around, so someone decided to test this. He got
>great results. Ordinary paraffin works good too, but the Paraplast
>is already in the form of pellets ready for use. It also has
>DMSO, which probably helps.
>(Usual disclaimers apply)
I have found that the addition of DMSO generally increases the yeild
of a PCR reaction, as well as the specificity. I do it in 10% DMSO final
concentration (high quality spectragrade, kept in aliquots at -80).
Previously, it would take 40 cycles to get my product. With DMSO,
a good band appears in only 20.
Well, PCR Gems help too :)
Richard Sucgang -- rs54 at cunixf.cc.columbia.edu
sucgang at cuhhca.hhmi.columbia.edu
"I was ten, I was blue, but I knew!" - A. Menken, Weird Romance
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