ethanol precipitation

Robert Coelen robert at arbo.microbiol.uwa.oz.au
Thu Jul 16 22:39:24 EST 1992


In article <1992Jul16.173410.25097 at gserv1.dl.ac.uk> skspoidn at uk.ac.rdg.susssys1 (Mike Poidinger) writes:
>wrt EtOh pptn and AmOAc salting, 
>
>Am ions severely inhibit polynucleotide kinase, 
>
>are there any other salts which have similar effects
> on other commonly used enzymes? I include in this 
>category salts which co ppt with the DNA and thence inhibit enzymes.
>
>BTW, I only ever use NaAc for ppting DNA
>
>Mike Poidinger
>Dept of Microbiology
>University of Reading
>
>"Spam, spam, spam, kits and spam"
>
> 
One of the advantages of using ammonium acetate is that it assist in 
removal of single nucleotides (and I think also small oligos). 
Certainly 2 successive etoh pptns with amm OAc as salt will remove in
excess of 95% of single nucleotides.

Robert Coelen
Dept of Microbiology
UWA

"Yuk, who would eat spam !"



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