Bill Melchior, NCTR/FDA
WMELCHIOR at NTET.NCTR.FDA.GOV
Thu Jul 16 09:21:47 EST 1992
>The problem I have with the use of ammonium acetate is the
>requirement for the addition of two volumes of salt in addition to
>the ethanol. This usually screws up the maximum volume that I can
>handle in a 1.5 ml tube. Any suggestions for avoiding this, like
>using higher concentration of amm. acetate?
This can be a problem. My procedure is to add 1/2 volume of 7.5 M
AmOAc, followed by twice the NEW volume of EtOH, giving a final volume
4 1/2 times as great as that of the original DNA solution. If anyone has
had experience using an even higher salt concentration, I too would like
to hear it.
The opinions stated are mine, not those of NCTR or its sponsoring organizations.
Bill Melchior || "You have lawyers the way
National Center for Toxicological Research || other people have mice."
Jefferson, AR 72079 ||
(501) 543-7206 || -Kenneth Duncan, English
|| Health & Safety Executive,
WMELCHIOR at NTDOC.NCTR.FDA.GOV || to US regulators
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