PCR of large fragments?

K_GARSON at UK.AC.ICRF K_GARSON at UK.AC.ICRF
Wed Jul 15 03:25:00 EST 1992


Path: wheel!k_garson
From: k_garson at uk.ac.icrf
Newsgroups: biosci.methods-and-reagents
Subject: PCR of large fragments?
Message-ID: <45637 at uk.ac.icrf>
Date: 15 Jul 92 09:24:34 GMT
Organization: Imperial Cancer Research Fund, London, UK
Lines: 9

I imagine that this has been covered in the past, but I would
like to know what parameters to change in PCR to acheive the
amplification of larger fragments (greater than 5 kb). Any suggestions
will be appreciated.

Ken Garson
ICRF
Lonon, UK
k_garson at icrf.ac.uk



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