southerns and electroblotting

Philip Joseph Moos moosp at matt.ksu.ksu.edu
Tue Jul 14 19:13:05 EST 1992


Hello,

	I'm wondering if anybody has a 'tried and true' protocol that uses
electroblotting for the transfer process in southern analysis instead of 
capillary transfer.  We have a Hoefer semi-dry blotter and there is an 
"experimental" method listed as an appendix in the manual but I would be 
interested in one that someone knows that it works.  I am planning on 
transfering plasmids and restriction digestions of plasmids so the DNA size 
is small (<5 kb).

Thanks in advance,

Philip Moos
moosp at matt.ksu.ksu.edu   



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