RAPD PCR sequencing

Jack Kramer kramer at umigw.miami.edu
Sat Jul 11 06:14:24 EST 1992


Has anyone done direct PCR sequencing of fragments extracted from
RAPD gels?  In normal PCR different primers are used at the 2 ends
of the amplimer.  One of the primers can be selected and labeled
to sequence one sense strand.  In Random Amplification of Polymorphic 
DNA only a single primer is used at both ends.  Labeled extension in only 
one sense is needed for the sequencing. No sequence information is known
for the amplimers.

Jack Kramer
Tropical Research and Education Center
University of Florida



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