PCR enzymes

Paul N Hengen pnh at fcsparc6.ncifcrf.gov
Thu Jul 9 15:42:26 EST 1992

In article <8JUL92.11400230 at wums.wustl.edu> wetsel_r at wums.wustl.edu writes:

>Greetings!  The delta Taq was actually developed here at Washington University
>by Dr. Wanyne Barnes.  His common name for it is Klen-Taq and he
>exclusively uses it for PCR. He'll often use upwards of 50U in a
>reaction for long PCRs. Like try lengths of 17kb!!!
>I personally have obtained 5 kb PCR fragments using the enzyme.

Rumor around here has it that someone at Johns Hopkins University has
used Hot-Tub to clone a 20 kb PCR product. Is this the record?
If this is true, would someone like to share the method?


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