AWS4270%USA.decnet at USAV01.GLAXO.COM
Thu Jul 9 08:25:00 EST 1992
I performed conjugal matings between lactic acid bacteria for my
graduate work. All you do is mix the donor and recipient at various
ratios, vortex, and collect on a filter. Incubate the filter cell
side up O/N at 37 C. Next day, stuff the filter in a sln and vortex,
plate on selective medium! A reference for my technique:
Shrago, AW et al. Applied and Environ. Microbiol. 1986. Vol. 52
Number 3, p. 574.
You will find additional references in the lit. cited!
Hope this helps!!!
Anne S. Howe
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