filter mating

USA::AWS4270 AWS4270%USA.decnet at USAV01.GLAXO.COM
Thu Jul 9 08:25:00 EST 1992


I performed conjugal matings between lactic acid bacteria for my
graduate work.  All you do is mix the donor and recipient at various
ratios, vortex, and collect on a filter.  Incubate the filter cell
side up O/N at 37 C.  Next day, stuff the filter in a sln and vortex,
plate on selective medium!  A reference for my technique:

Shrago, AW et al.  Applied and Environ. Microbiol. 1986. Vol. 52
Number 3, p. 574.

You will find additional references in the lit. cited!

Hope this helps!!!

Anne S. Howe




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