Any opinions on Magic Minipreps(tm)
Ramaswamy K Iyer
gene at bach.udel.edu
Fri Jul 3 16:45:42 EST 1992
I have been using the Magic miniprep columns for a while now.
The DNA you get out of this is very clean (even from endA+ host strains), and
works beautifully in all the different reactions I have tried (restriction
digestion, cloning, sequencing, PCR etc.). In fact we like it so much that
we use it exclusively. I have scaled it up successfully to obtain large (1-2ug)of clean DNA.
I should mention that I only buy the mini-columns and the DNA
binding resin, and make up the solutions for the alkaline lysis myself (they
formulations are in the literature provided with the kit).
I am also using the binding resin to obtain very good quality
single stranded DNa for sequencing and oligonucleotide-directed, site-specific
mutagenesis by Kunkel's method.
I know that some people do not like kits, but I love this one.
Ramaswamy K. Iyer
P.S. I am not an employee nor do I have any commercial interest in Promega.
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