pUC13 sequence

Paul Walden walden at mcclb0.med.nyu.edu
Mon Jun 29 18:17:16 EST 1992


In article <01GLS6B9PB6O8WW39O at epavax.rtpnc.epa.gov>, MASS at herlvx.rtpnc.epa.gov ("Marc J. Mass") writes:
> Can anyone send the nucleotide sequence of pUC13 to me?
> 
> Many thanks,
> 
> MJ Mass, Ph.D.

Here is the sequence of pUC13 from Vecbase.

*****************************************************************************
Paul Walden, Ph.D.
Department of Biochemistry
550 First Avenue
New York University Medical Center
New York 
NY 10016                                  Internet: WALDEN at MCCLB0.MED.NYU.EDU
*****************************************************************************

DC;PUC13      - pUC13 - Cloning vector
ENTRY PUC13                    #TYPE DNA CIRCULAR
TITLE pUC13 - Cloning vector
DATE      17-SEP-1986
   #sequence  16-DEC-1986
ACCESSION VB0024
SOURCE    artificial
REFERENCE
   #number  1
   #authors  Vieira J., Messing J.
   #journal Gene (1982) 19: 259-268
   #comment see "note added in proof"
REFERENCE
   #number 2
   #authors Pouwels P.H., Enger-Valk B.E., Brammar W.J.
   #book    Cloning Vectors, Elsvier 1985 and supplements
   #comment  vector I-A-iv-20
COMMENT
   Assembled from pUC19 and M13mp11 by F. Pfeiffer, MPI, Martinsried
   Revised 16-DEC-1986 by F. Pfeiffer:
   2302/3 "AT" to "TA" to match revised sequence of PBR322
   The strand shown contains the beta-galactosidase mRNA sequence
   including the multiple cloning site of M13mp11.
KEYWORDS
CROSSREFERENCE
   #complement
     VecBase(3):pUC13c
   #parent
     VecBase(3):pUC9, VecBase(3):M13mp11, VecSource(3):bGal13
   #brother
     VecBase(3):pUC12
   #offspring
     VecBase(3):pUC19, VecBase(3):pUC19c,
     VecBase(3):pSP65, VecBase(3):pGEM2, VecBase(3):pT713
PARENT
   Features of pUC13 (2680 bp)
     residue     source
      1- 441  6002-6442      M13mp11
      1- 230  1069-1298      Lac-Operon
    233- 283     1-  51      polylinker of M13mp11
    284- 441  1304-1461      Lac-Operon
    442- 541  2351-2252  (c) pBR322
    542- 678  2210-2074  (c) pBR322
    679-2680  4355-2354  (c) pBR322
   Conflict (cfl) and Mutations (mut):
        pUC13  source
   mut 1122 T  C 3912 (c) pBR322
   mut 1423 A  G 3611 (c) pBR322
FEATURE
   946-1734 1-789 Ap-R; b-lactamase
POLYLINKER  HindIII-PstI-SalI-XbaI-BamHI-SmaI-SacI-EcoRI
SELECTION
   #resistance  Ap
   #indicator beta-galactosidase
SUMMARY  pUC13    #length 2680   #checksum 217
SEQUENCE

   Puc13  Length: 2680  June 29, 1992  18:12  Type: N  Check: 217  ..

       1  GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT 

      51  GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC 

     101  GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT 

     151  TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC 

     201  ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTTGG GCTGCAGGTC 

     251  GACTCTAGAG GATCCCCGGG CGAGCTCGAA TTCACTGGCC GTCGTTTTAC 

     301  AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA 

     351  GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA 

     401  TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCCTGATGC 

     451  GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC 

     501  ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA 

     551  CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT 

     601  CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG 

     651  TTTTCACCGT CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG 

     701  CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG 

     751  GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC 

     801  TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT 

     851  GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG 

     901  TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC 

     951  CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG 

    1001  AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 

    1051  TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA 

    1101  TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG 

    1151  CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG 

    1201  AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC 

    1251  ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG 

    1301  AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 

    1351  CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC 

    1401  GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT 

    1451  ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT 

    1501  GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG 

    1551  GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG 

    1601  CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 

    1651  TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG 

    1701  ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA 

    1751  AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA 

    1801  AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT 

    1851  TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 

    1901  AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 

    1951  CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA 

    2001  CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 

    2051  TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG 

    2101  TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT 

    2151  GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 

    2201  ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 

    2251  CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG 

    2301  CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 

    2351  GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG 

    2401  GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT 

    2451  GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 

    2501  CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 

    2551  CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT 

    2601  ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 

    2651  CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 



More information about the Methods mailing list