PCR enzymes
Joel A. Kreps
jak8 at po.CWRU.Edu
Tue Jun 23 08:14:08 EST 1992
Dear Network, 6.23.92
I am shopping for PCR enzymes. I have received a lot of literature
from various companies telling me all about errors rates, process.
etc. I posted a query earlier on this network and received some
very useful info along these lines. I plan on using PCR to
amplify cDNA and then cloning the product for sequencing. I have
some enzyme which I can use, it is called Pyrostase from Molec.
Genetic Resources. Does anyone have any experience with this
enzyme? Are there any enzymes I should stay away from (such
as Vent, which I have been told leaves ragged ends which are un-
suitable for RE digest and cloning)? Any helpful hints will be
appreciated.
Sincerely,
Joel A. Kreps
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