PCR enzymes

[Francis Ouellette]

Joel A. Kreps  (jak8 at po.CWRU.Edu) writes:


>I am shopping for PCR enzymes.  I have received a lot of literature
.
.

> Are there any enzymes I should stay away from (such
> as Vent, which I have been told leaves ragged ends which are un-
> suitable for RE digest and cloning)?  

Dear Joel,

if I can come to the defence of NEB (and no, I do not work for them)
they have two different Vent, one with exonuclease correcting activity, 
and onw which doesn't.  I assume the exo+ enzyme would not leave ragged 
ends.  I also know (from a friend/colleague) that tried to do some in 
vitro mutagenesis with an 30mer, and ssDNA and vent (exo+) and the 
enzyme chewed back and corrected the oligo to remove the mutation ... 
the exo activity works!

regards,

francis


---
| B.F. Francis Ouellette  
| manager, yeast chromosome I project
| dept of biology, McGill university, Montreal, Qc, Canada
| francis at monod.biol.mcgill.ca