Direct sequencing PCR products ?

Bruce Roe broe at aardvark.ucs.uoknor.edu
Fri Jun 19 11:30:00 EST 1992


In article <1992Jun19.142838.2800 at crc.ac.uk>, saparici at crc.ac.uk (Dr. S.A.J.R. Aparicio) writes...
>Of course!
> 
>We sequecnce double stranded PCR products on an ABI 373A and
>can get 400-450 bp reliable sequence
> 
>Sam Aparicio

Sam, Sam, Sam,
	Let's not open this pandora's box again, but 

	1.  please post your isolation protocol for everyone
	    because the key is getting pure,clean template, and
	2.  please define reliable.
Cheers....bruce



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