Tn5 sequence

PAU at UK.AC.AFRC.NFL PAU at UK.AC.AFRC.NFL
Fri Jun 12 10:08:00 EST 1992


>I have been searching for a recent Tn5 restriction map, or its sequence,
>without much success.  I would be extremely grateful for any assistance in this
>regard.

Here is the Tn5 sequence which we assembled from segments on the database.
I hope its useful, but I can give no guarantee as to its accuracy. It is
in UWGCG format with the headers included so that the sources and joins
can be seen.
Richard Pau, Nitrogen Fixation Laboratory, Brighton, Sussex.
Email PAU at UK.AC.AFRC.NFL
--------------------------------------------------------------------

>DL;TRN5IR1
Transposon Tn5 left inverted repeat with genes for transposase and
 Km-resistance (aminoglycoside-3'-O-phosphotransferase)
LOCUS       TRN5IR1      1737 bp ds-DNA             BCT       15-DEC-1988
DEFINITION  Transposon Tn5 left inverted repeat with genes for transposase and
            Km-resistance (aminoglycoside-3'-O-phosphotransferase).
ACCESSION   V00615
KEYWORDS    aminoglycoside-3'-O-phosphotransferase; drug resistance;
            insertion sequence; kanamycin resistance; transposase.
SEGMENT     1 of 2
SOURCE      Transposon Tn5 DNA [2],[1].
  ORGANISM  Transposon Tn5
            Prokaryota; Bacteria; Transposon Tn5.
REFERENCE   1 (bases 1 to 100)
  AUTHORS   Fuller,R.S., Funnell,B.E. and Kornberg,A.
  TITLE     The dnaA protein complex with the E. coli chromosomal replication
            origin (oriC) and other DNA sites
  JOURNAL   Cell 38, 889-900 (1984)
  STANDARD  full staff_review
REFERENCE   2 (bases 38 to 1737)
  AUTHORS   Auerswald,E.-A., Ludwig,G. and Schaller,H.
  TITLE     Structural analysis of Tn5
  JOURNAL   Cold Spring Harb. Symp. Quant. Biol. 45, 107-113 (1981)
  STANDARD  full staff_review
COMMENT     Even though an unidentified reading frame is indicated in [2], a
            protein of the correct size coded for by the sequence shown here
            has been identified.  The protein is believed to be a transposase.
FEATURES             Location/Qualifiers
     CDS             129. .1481
                     /note="transposase"
     CDS             1588. .>1737
                     /note="aminoglycoside-3'-O-phosphotransferase"
     repeat_region   38. .1571
                     /note="left inverted repeat"
     misc_binding    45. .53
                     /note="dnaA binding site [Cell 38, 889-900 (1984)]"
BASE COUNT      421 a    445 c    515 g    356 t
ORIGIN      HaeIII site.

    tn5.seq  Length: 5865  November 21, 1990  16:37  Check: 517  ..

       1  CCAGCAAGCA AGCTAAAAAG TAAAGCAACA ACATAACCTG ACTCTTATAC 

      51  ACAAGTAGCG TCCTGAACGG AACCTTTCCC GTTTTCCAGG ATCTGACTTC 

     101  CATGTGACCT CCTAACATGG TAACGTTCAT GATAACTTCT GCTCTTCATC 

     151  GTGCGGCCGA CTGGGCTAAA TCTGTGTTCT CTTCGGCGGC GCTGGGTGAT 

     201  CCTCGCCGTA CTGCCCGCTT GGTTAACGTC GCCGCCCAAT TGGCAAAATA 

     251  TTCTGGTAAA TCAATAACCA TCTCATCAGA GGGTAGTGAA GCCATGCAGG 

     301  AAGGCGCTTA CCGATTTTAC CGCAATCCCA ACGTTTCTGC CGAGGCGATC 

     351  AGAAAGGCTG GCGCCATGCA AACAGTCAAG TTGGCTCAGG AGTTTCCCGA 

     401  ACTGCTGGCC ATTGAGGACA CCACCTCTTT GAGTTATCGC CACCAGGTCG 

     451  CCGAAGAGCT TGGCAAGCTG GGCTCTATTC AGGATAAATC CCGCGGATGG 

     501  TGGGTTCACT CCGTTCTCTT GCTCGAGGCC ACCACATTCC GCACCGTAGG 

     551  ATTACTGCAT CAGGAGTGGT GGATGCGCCC GGATGACCCT GCCGATGCGG 

     601  ATGAAAAGGA GAGTGGCAAA TGGCTGGCAG CGGCCGCAAC TAGCCGGTTA 

     651  CGCATGGGCA GCATGATGAG CAACGTGATT GCGGTCTGTG ACCGCGAAGC 

     701  CGATATTCAT GCTTATCTGC AGGACAGGCT GGCGCATAAC GAGCGCTTCG 

     751  TGGTGCGCTC CAAGCACCCA CGCAAGGACG TAGAGTCTGG GTTGTATCTG 

     801  ATCGACCATC TGAAGAACCA ACCGGAGTTG GGTGGCTATC AGATCAGCAT 

     851  TCCGCAAAAG GGCGTGGTGG ATAAACGCGG TAAACGTAAA AATCGACCAG 

     901  CCCGCAAGGC GAGCTTGAGC CTGCGCAGTG GGCGCATCAC GCTAAAACAG 

     951  GGGAATATCA CGCTCAACGC GGTGCTGGCC GAGGAGATTA ACCCGCCCAA 

    1001  GGGTGAGACC CCGTTGAAAT GGTTGTTGCT GACCGGCGAA CCGGTCGAGT 

    1051  CGCTAGCCCA AGCCTTGCGC GTCATCGACA TTTATACCCA TCGCTGGCGG 

    1101  ATCGAGGAGT TCCATAAGGC ATGGAAAACC GGAGCAGGAG CCGAGAGGCA 

    1151  ACGCATGGAG GAGCCGGATA ATCTGGAGCG GATGGTCTCG ATCCTCTCGT 

    1201  TTGTTGCGGT CAGGCTGTTA CAGCTCAGAG AAAGCTTCAC GCTGCCGCAA 

    1251  GCACTCAGGG CGCAAGGGCT GCTAAAGGAA GCGGAACACG TAGAAAGCCA 

    1301  GTCCGCAGAA ACGGTGCTGA CCCCGGATGA ATGTCAGCTA CTGGGCTATC 

    1351  TGGACAAGGG AAAACGCAAG CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG 

    1401  GCTTACATGG CGATAGCTAG ACTGGGCGGT TTTATGGACA GCAAGCGAAC 

    1451  CGGAATTGCC AGCTGGGGCG CCCTCTGGTA AGGTTGGGAA GCCCTGCAAA 

    1501  GTAAACTGGA TGGCTTTCTT GCCGCCAAGG ATCTGATGGC GCAGGGGATC 

    1551  AAGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG 

    1601  ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 

    1651  TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 

    1701  GCTGTCAGCG CAGGGGCGCC GGTTCTTTTT GTCAAGA
                                                  >SEQED 
          (include) of: trn5ne.gcg check: 3912 from: 302 to: 
          1300>
                                                  CCG ACCTGTCCGG 

    1751  TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA 

    1801  CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 

    1851  AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC 

    1901  TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC 

    1951  GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 

    2001  CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA 

    2051  GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG 

    2101  CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT 

    2151  GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG 

    2201  ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG 

    2251  CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 

    2301  CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC 

    2351  CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA 

    2401  AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 

    2451  CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC 

    2501  GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA 

    2551  CCCCGGGCTC GATCCCCTCG CGAGTTGGTT CAGCTGCTGC CTGAGGCTGG 

    2601  ACGACCTCGC GGAGTTCTAC CGGCAGTGCA AATCCGTCGG CATCCAGGAA 

    2651  ACCAGCAGCG GCTATCCGCG CATCCATGCC CCCGAACTGC AGGAGTGGGG 

    2701  AGGCACGATG GCCGCTTTGG TCGACCCGGA CGGGA
                                                <SEQED 
          (include) of: trn5ne.gcg check: 3912 from: 302 to: 
          1300<
                                                C
                                                 >SEQED 
          (include) of: TRN5STR.GCG check: 9384 from: 390 to: 
          2040>
                                                 CCGG ACGGGACGCT 

    2751  CCTGCGCCTG ATACAGAACG AATTGCTTGC AGGCATCTCA TGAGTGTGTC 

    2801  TTCCCGTTTT CCGCCTGAGG TCACTGCGTG GATGGAGCGC TGGCGCCTGC 

    2851  TGCGCGACGG CGAGCTGCTC ACCACCCACT CGAGCTGGAT ACTTCCCGTC 

    2901  CGCCAGGGGG ACATGCCGGC GATGCTGAAG GTCGCGCGCA TTCCCGATGA 

    2951  AGAGGCCGGT TACCGCCTGT TGACCTGGTG GGACGGGCAG GGCGCCGCCC 

    3001  GAGTCTTCGC CTCGGCGGCG GGCGCTCTGC TCATGGAGCG CGCGTCCGGG 

    3051  GCCGGGGACC TTGCACAGAT AGCGTGGTCC GGCCAGGACG ACGAGGCTTG 

    3101  CAGGATCCTC TGCGACACCG CCGCTCGTCT GCACGCGCCG CGGTCCGGAC 

    3151  CGCCGCCCGA TCTCCATCCG CTACAGGAAT GGTTCCAGCC GCTTTTCCGG 

    3201  TTGGCCGCTG AGCACGCGGC ACTTGCGCCC GCCGCCAGCG TAGCGCGCCA 

    3251  ACTTCTGGCG GCGCCGCGCG AGGTGTGCCC GCTCCACGGC GACCTGCACC 

    3301  ACGAGAACGT GCTCGACTTC GGCGACCGCG GCTGGCTGGC CATCGACCCG 

    3351  CACGGACTGC TCGGCGAGCG CACCTTCGAC TATGCCAACA TCTTCACGAA 

    3401  TCCCGATCTC AGCGACCCCG GTCGCCCGCT TGCGATCCTG CCGGGCAGGC 

    3451  TGGAGGCTCG ACTCAGCATT GTGGTCGCGA CGACCGGGTT TGAGCCCGAA 

    3501  CGGCTTCTTC GCTGGATCAT TGCATGGACG GGCTTGTCGG CAGCCTGGTT 

    3551  CATCGGCGAC GGCGACGGCG AGGGCGAGGG CGCTGCGATT GATCTGGCCG 

    3601  TAAACGCCAT GGCACGCCGG TTGCTTGACT AGCGCGGTCA CCGATCTCAC 

    3651  CTGGTCGTCG AGCTAGGTCA GGCCGTGTCG GGCGTGATCC GCTGGAAGTC 

    3701  GTTGCGGGCC ACACCCGCCG CCTCGAAGCC CTGCACCAGG CCGGCATCGT 

    3751  GGTGTGCGTG GCCGAGGGAC TATGGAAGGT GCCGGACGAT CTGCCCGAGC 

    3801  AGGGCCGCCG CTATGACGCC CAGCGTCTTG GTGGCGTGAC GGTGGAGCTG 

    3851  AAATCGCACC TGCCCATCGA GCGGCAGGCC CGCGTGATCG GTGCCACCTG 

    3901  GCTTGACCAG CAGTTGATCG ACGGTGGCTC GGGCTTGGGC GACCTGGGCT 

    3951  TTAGCAGTGA GGCCAAGTAG GCGATACAGC AGCGCGCGGA CTTCCTGGCC 

    4001  GAACAGGGAC TGGCCGAGCG GCGCGGGCAG CGCGTGATCC TCACCGGAAT 

    4051  CTGCTCGGCA GCAGCGGGCT CGGGAACTGG CGCAGGCCGC GAAGGACATT 

    4101  GCCGCCGATA CCGGCCTGGA GCATCGCCCC GTGGCCGACG GCCAGCGCGT 

    4151  TGCCGGCGTC TACCGGCGCC CCGTCATGCT CGCCAGCGGG CGAAATGGGA 

    4201  TGCTTGATGA CGCCAAGGGG TCCAGCCTCG TGCGGTGGAA GCCCATCGAA 

    4251  CAGCGGCTTG GGGAGCAGCT CGCCGCGACG GTGCGCGGTG GCGGCGTGTC 

    4301  TTGGGAGATT GGACGACAGC GTGGGCCGGC CCCTGTCTCT TGATCAGATC 

    4351  TTGATCCCCT GCGCCATCAG ATCCTTGGCG GCAAGAA
                                                  <SEQED 
          (include) of: TRN5STR.GCG check: 9384 from: 390 to: 
          2040<
                                                  >SEQED 
          (include) of:  TN5R.SEQ  check:  6145  from:  123  
          to: 1600>
                                                  AGC CATCCAGTTT 

    4401  ACTTTGCAGG GCTTCCCAAC CTTCCCAGAG GGCGCCCCAG CTGGCAATTC 

    4451  CGGTTCGCTT GCTGTCCATA AAACCGCCCA GTCTAGCTAT CGCCATGTAA 

    4501  GCCCACTGCA AGCTACCTGC TTTCTCTTTG CGCTTGCGTT TTCCCTTGTC 

    4551  CAGATAGCCC AGTAGCTGAC ATTCATCCGG GGTCAGCACC GTTTCTGCGG 

    4601  ACTGGCTTTC TACGTGTTCC GCTTCCTTTA GCAGCCCTTG CGCCCTGAGT 

    4651  GCTTGCGGCA GCGTGAAGCT TTCTCTGAGC TGTAACAGCC TGACCGCAAC 

    4701  AAACGAGAGG ATCGAGACCA TCCGCTCCAG ATTATCCGGC TCCTCCATGC 

    4751  GTTGCCTCTC GGCTCCTGCT CCGGTTTTCC ATGCCTTATG GAACTCCTCG 

    4801  ATCCGCCAGC GATGGGTATA AATGTCGATG ACGCGCAAGG CTTGGGCTAG 

    4851  CGACTCGACC GGTTCGCCGG TCAGCAACAA CCATTTCAAC GGGGTCTCAC 

    4901  CCTTGGGCGG GTTAATCTCC TCGGCCAGCA CCGCGTTGAG CGTGATATTC 

    4951  CCCTGTTTTA GCGTGATGCG CCCACTGCGC AGGCTCAAGC TCGCCTTGCG 

    5001  GGCTGGTCGA TTTTTACGTT TACCGCGTTT ATCCACCACG CCCTTTTGCG 

    5051  GAATGCTGAT CTGATAGCCA CCCAACTCCG GTTGGTTCTT CAGATGGTCG 

    5101  ATCAGATACA ACCCAGACTC TACGTCCTTG CGTGGGTGCT TGGAGCGCAC 

    5151  CACGAAGCGC TCGTTATGCG CCAGCCTGTC CTGCAGATAA GCATGAATAT 

    5201  CGGCTTCGCG GTCACAGACC GCAATCACGT TGCTCATCAT GCTGCCCATG 

    5251  CGTAACCGGC TAGTTGCGGC CGCTGCCAGC CATTTGCCAC TCTCCTTTTC 

    5301  ATCCGCATCG GCAGGGTCAT CCGGGCGCAT CCACCACTCC TGATGCAGTA 

    5351  ATCCTACGGT GCGGAATGTG GTGGCCTCGA GCAAGAGAAC GGAGTGAACC 

    5401  CACCATCCGC GGGATTTATC CTGAATAGAG CCCAGCTTGC CAAGCTCTTC 

    5451  GGCGACCTGG TGGCGATAAC TCAAAGAGGT GGTGTCCTCA ATGGCCAGCA 

    5501  GTTCGGGAAA CTCCTGAGCC AACTTGACTG TTTGCATGGC GCCAGCCTTT 

    5551  CTGATCGCCT CGGCAGAAAC GTTGGGATTG CGGTAAAATC GGTAAGCGCC 

    5601  TTCCTGCATG GCTTCACTAC CCTCTGATGA GATGGTTATT GATTTACCAG 

    5651  AATATTTTGC CAATTGGGCG GCGACGTTAA CCAAGCGGGC AGTACGGCGA 

    5701  GGATCACCCA GCGCCGCCGA AGAGAACACA GATTTAGCCC AGTCGGCCGC 

    5751  ACGATGAAGA GCAGAAGTTA TCATGAACGT TACCATGTTA GGAGGTCACA 

    5801  TGGAAGTCAG ATCCTGGAAA ACGGGAAAGG TTCCGTTCAG GACGCTACTT 

    5851  GTGTATAAGA GTCAG

                          <SEQED (include) of: TN5R.SEQ  check: 
          6145  from: 123  to: 1600<



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