separable subunits of a functionnal enzyme

Keith Robison robison1 at
Thu Jun 11 07:51:14 EST 1992

verwaerde at writes:

>Does any one know any enzyme which has two separable units.
>When you bring those units together you'll induce an in vivo functionnal assay
>like beta-galactosidase or luciferase.

>Phil Verwaerde, CABM, Rutgers university

Your post mentions one of the best examples.  Those blue-white colony
assays work because your plasmid vector carries the coding region for
a small peptide and the E.coli host the rest of beta-galactosidase.

Keith Robison
Harvard University
Program in Biochemistry, Molecular, Cellular, and Developmental Biology

robison at 

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