separable subunits of a functionnal enzyme

Keith Robison robison1 at husc9.harvard.edu
Thu Jun 11 07:51:14 EST 1992


verwaerde at mbcl.rutgers.edu writes:

>Does any one know any enzyme which has two separable units.
>When you bring those units together you'll induce an in vivo functionnal assay
>like beta-galactosidase or luciferase.
      ^^^^^^^^^^^^^^^^^^
>Thanks

>Phil Verwaerde, CABM, Rutgers university

Your post mentions one of the best examples.  Those blue-white colony
assays work because your plasmid vector carries the coding region for
a small peptide and the E.coli host the rest of beta-galactosidase.



Keith Robison
Harvard University
Program in Biochemistry, Molecular, Cellular, and Developmental Biology

robison at ribo.harvard.edu 



More information about the Methods mailing list