separable subunits of a functionnal enzyme
robison1 at husc9.harvard.edu
Thu Jun 11 07:51:14 EST 1992
verwaerde at mbcl.rutgers.edu writes:
>Does any one know any enzyme which has two separable units.
>When you bring those units together you'll induce an in vivo functionnal assay
>like beta-galactosidase or luciferase.
>Phil Verwaerde, CABM, Rutgers university
Your post mentions one of the best examples. Those blue-white colony
assays work because your plasmid vector carries the coding region for
a small peptide and the E.coli host the rest of beta-galactosidase.
Program in Biochemistry, Molecular, Cellular, and Developmental Biology
robison at ribo.harvard.edu
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