Western Blotting

verwaerde at mbcl.rutgers.edu verwaerde at mbcl.rutgers.edu
Mon Jun 8 15:46:21 EST 1992

In article <9205261723.AA19059 at shiva.psu.edu>, guiltin at SHIVA.PSU.EDU (Dr. Mark Guiltinan) writes:
>      Does anyone have suggestions for optimizing our westerns?  We are using immobilonP, and the Millipore semi-dry blotter as specified by thet seem to have either poor transfer or low binding of our protein (25 kD, pI 8.0).  Thanks for any help.
You should try to include SDS 0.1% in your buffers.
I use immobilon and semi dry (millipore) without any problems.
the pI=8 may explain your problem.
Phil Verwaerde CABM Rutgers University

> Mark Guiltinan
> Penn State Biotechnology Institute

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