20X TBE without ppt?
num208jn at MBDS.NRC.CA
Tue Jun 9 07:54:08 EST 1992
In article <92161.002546GERLACHJ at QUCDN.QueensU.CA>, Jim Gerlach <GERLACHJ at QUCDN.QueensU.CA> writes:
>My grad student recalls an article that told how to make 20X TBE stock without
>having it precipitate within a short while. She thinks it required changing
>pH slightly. Does anyone have info on this or any other recommendations for
>solving the precipitation problem?
>Thanx in advance, Jim
I remember the article, but not the reference. What I do is make it
but I don't pH it (it comes out to approx 7.8 in my hands). Then I
put it through a (Nalgene) .45 um filter. Apparently dust and stuff
causes it to precipitate, and filtration reduces that likelihood. My
20X stock will last for months if I remove TBE stock with a pipette.
When I pour it out of the bottle, I have to be careful -if the crust
which forms on the rim of the bottle falls back in, it causes the
buffer to get the precipitate ;-(
John Nash (Internet:) Nash at biologysx.lan.nrc.ca.
Email to my other addresses is forwarded automatically,
Disclaimer: All opinions are mine, not NRC's!
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