Cloning PCR products

Michael Benedik bchs1b at Elroy.UH.EDU
Thu Jun 4 21:54:14 EST 1992


In article <1992Jun3.160305.12666 at crc.ac.uk>, naffara at crc.ac.uk (Dr. N.A. Affara) writes:
>This is a repeat of an earlier message which I think didn't get through,
>I apologise if you have seen this before.
>Has anyone tried to cut a DNA fragment (not a PCR product) with an enzyme
>which is near to the end e.g. up to 25 bases away. I am interested to
>find out if the problem cutting PCR products is because the site is too
>close to the end or something else entirely.
>
>Nabeel


I think it is the New England Biolabs catalog that shows the efficiency
of digestion for some different enzymes with sites very near the ends.
I believe they used linkers. The verdict is that in general you get
very efficient cutting with only a few extra bases (like 2-4).
	---------------------------------------------------
	 Michael Benedik
	 Department of Biochemical and Biophysical Sciences
	 University of Houston
	 INTERNET: Benedik at UH.EDU	BITNET: Benedik at UHOU



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