Spin columns

rybicki at uctvax.uct.ac.za rybicki at uctvax.uct.ac.za
Wed Jun 3 10:13:07 EST 1992


In article <1992May28.173320.9941 at rockyd.rockefeller.edu>, verland at rockyj.rockefeller.edu (Peter Verlander) writes:
> In article <19594.9205210828 at subnode.susssys1.rdg.ac.uk> skspoidn at uk.ac.rdg.susssys1 (Mike Poidinger) writes:
> 
>>We make spin columns like so:
>>1) Cut the lids of one normal and one small eppendorf
>>2) put a hole in the bottom of the small eppendorf with a 23 gauge needle
>>3) Put 100ul glass beads in the small tube, then fill with Sepharose CL6B
>>4) Put the little tube into the big one
> 
>>voila!
> 
> The easiest way I've found to make these columns is to use eppendorf
> tubes with a filter insert (eg SpinX tubes from CoStar) - just add 
> your favorite gel to the 'upper chamber' - no needles or glass beads
> to mess around with.
> 
> Pete Verlander
> Rockefeller University
-- 
It's easier and cheaper to fool with a little and a big eppi if you don't have
access to some of these nice little filter inserts (which I've never even
heard of) - and to use siliconised glass wool rather than a glass bead.  Even
simpler is the Pasteur column - glass bead and Sephadev slurry, gravity elute,
set up a line of 'em and just add eluant; surface tension stops 'em running
dry, so you can fractionate a whole bunch of things simultaneously without
a microfuge - and as you collect fractions, you can make judgement calls as
to which to pool and which to toss.
 _________________________________________________________________________
|  Ed Rybicki                     |    Now you've got the hang of it      |
|  Dept Microbiology              | There's nothing you can't do with it  |
|  University of Cape Town        |         If you're very into it        |
|  PB Rondebosch 7700             |         You can't go wrong....        |
|  South Africa                   |                                       |
|  ed at micro.uct.ac.za             |              - Mad John               |
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