RNAse Protection Assay

kim at m44.unm.edu kim at m44.unm.edu
Wed Jun 3 10:48:17 EST 1992


Hello Group:

I read a paper in BioTechniques of May 1992 by Haines and Gillespie concerning
RNAse Protection assays done using crude cell lysates in Guanidine thiocyanate. 
There were a couple of related articles in Analytical Biochemistry cited as
well.  This seems like an interesting and time-saving way of doing this
procedure, and now a kit is available as well.

Has anyone tried this method?  Does it really work?

Daniel Kim

P.S.  re: a comment made in another posting about using kits:  Sometimes they
are a nice way to overcome intimidation at a new procedure.



More information about the Methods mailing list